vector pj98 (Addgene inc)
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Addgene inc
vector pj98
Vector Pj98, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cmv+brainbow+2+1+r/CMV-Brainbow-2%2E1+R+(Plasmid+%2318723)/pmc12509891-376-14-16
Average 93 stars, based on 20 article reviews
Vector Pj98, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cmv+brainbow+2+1+r/CMV-Brainbow-2%2E1+R+(Plasmid+%2318723)/pmc12509891-376-14-16
Average 93 stars, based on 20 article reviews
vector pj98 - by Bioz Stars,
2026-09
93/100 stars
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Polymerase Chain Reaction:Article Title: Digital switching in a biosensor circuit via programmable timing of gene availability Article Snippet: .. Cerulean was PCR amplified with PR13 and PR14 from Article Title: Generation of a human embryonic stem cell line (SMUDHe010-A-1A) carrying Brainbow cassette in the AAVS1 gene by CRISPR/Cas9-mediated homologous recombination. Article Snippet: Firstly, the double strands of sgAAVS1 were annealed, introduced into lenti CRISPRv2 vector, which had been digested with BsmBI (NEB, USA, R0739L) before, using the T4 DNA Ligase (NEB, USA, M0202S), to get a plasmid named lenti-sgAAVS1. .. To construct the donor plasmid AAVS1-arm1-Brainbow-arm2, we firstly cloned two AAVS1 locus homology arms by PCR amplifying from the H9 cell DNA, and CMV enhancer, chicken β-actin promoter, chimeric intron fragments were amplified by PCR from Article Title: Digital switching in a biosensor circuit via programmable timing of gene availability Article Snippet: .. Cerulean was PCR amplified with PR13 and PR14 from Amplification:Article Title: Digital switching in a biosensor circuit via programmable timing of gene availability Article Snippet: .. Cerulean was PCR amplified with PR13 and PR14 from Article Title: Generation of a human embryonic stem cell line (SMUDHe010-A-1A) carrying Brainbow cassette in the AAVS1 gene by CRISPR/Cas9-mediated homologous recombination. Article Snippet: Firstly, the double strands of sgAAVS1 were annealed, introduced into lenti CRISPRv2 vector, which had been digested with BsmBI (NEB, USA, R0739L) before, using the T4 DNA Ligase (NEB, USA, M0202S), to get a plasmid named lenti-sgAAVS1. .. To construct the donor plasmid AAVS1-arm1-Brainbow-arm2, we firstly cloned two AAVS1 locus homology arms by PCR amplifying from the H9 cell DNA, and CMV enhancer, chicken β-actin promoter, chimeric intron fragments were amplified by PCR from Article Title: Digital switching in a biosensor circuit via programmable timing of gene availability Article Snippet: .. Cerulean was PCR amplified with PR13 and PR14 from Plasmid Preparation:Article Title: Digital switching in a biosensor circuit via programmable timing of gene availability Article Snippet: .. Cerulean was PCR amplified with PR13 and PR14 from Article Title: Diversity and wiring variability of visual local neurons in the Drosophila medulla M6 stratum Article Snippet: .. Article Title: Generation of a human embryonic stem cell line (SMUDHe010-A-1A) carrying Brainbow cassette in the AAVS1 gene by CRISPR/Cas9-mediated homologous recombination. Article Snippet: Firstly, the double strands of sgAAVS1 were annealed, introduced into lenti CRISPRv2 vector, which had been digested with BsmBI (NEB, USA, R0739L) before, using the T4 DNA Ligase (NEB, USA, M0202S), to get a plasmid named lenti-sgAAVS1. .. To construct the donor plasmid AAVS1-arm1-Brainbow-arm2, we firstly cloned two AAVS1 locus homology arms by PCR amplifying from the H9 cell DNA, and CMV enhancer, chicken β-actin promoter, chimeric intron fragments were amplified by PCR from Article Title: Diversity and wiring variability of visual local neurons in the Drosophila medulla M6 stratum Article Snippet: CMV-Brainbow-2.1 R (Livet et al., ) was purchased from Addgene (Cambridge, MA; plasmid 18723) and contains four cassettes of fluorescent protein (hrGFPII-NLS, eYFP, tdimer2, and M-mCerulean), each containing a loxp site linked to a fluorescent protein-coding region and a terminator. .. The Article Title: Development of an in vivo model to study clonal lineage relationships in hematopoietic cells using Brainbow2.1/Confetti mice Article Snippet: .. The four Brainbow2.1 XFPs were cloned from Article Title: Digital switching in a biosensor circuit via programmable timing of gene availability Article Snippet: .. Cerulean was PCR amplified with PR13 and PR14 from Construct:Article Title: Generation of a human embryonic stem cell line (SMUDHe010-A-1A) carrying Brainbow cassette in the AAVS1 gene by CRISPR/Cas9-mediated homologous recombination. Article Snippet: Firstly, the double strands of sgAAVS1 were annealed, introduced into lenti CRISPRv2 vector, which had been digested with BsmBI (NEB, USA, R0739L) before, using the T4 DNA Ligase (NEB, USA, M0202S), to get a plasmid named lenti-sgAAVS1. .. To construct the donor plasmid AAVS1-arm1-Brainbow-arm2, we firstly cloned two AAVS1 locus homology arms by PCR amplifying from the H9 cell DNA, and CMV enhancer, chicken β-actin promoter, chimeric intron fragments were amplified by PCR from Clone Assay:Article Title: Generation of a human embryonic stem cell line (SMUDHe010-A-1A) carrying Brainbow cassette in the AAVS1 gene by CRISPR/Cas9-mediated homologous recombination. Article Snippet: Firstly, the double strands of sgAAVS1 were annealed, introduced into lenti CRISPRv2 vector, which had been digested with BsmBI (NEB, USA, R0739L) before, using the T4 DNA Ligase (NEB, USA, M0202S), to get a plasmid named lenti-sgAAVS1. .. To construct the donor plasmid AAVS1-arm1-Brainbow-arm2, we firstly cloned two AAVS1 locus homology arms by PCR amplifying from the H9 cell DNA, and CMV enhancer, chicken β-actin promoter, chimeric intron fragments were amplified by PCR from Article Title: Diversity and wiring variability of visual local neurons in the Drosophila medulla M6 stratum Article Snippet: CMV-Brainbow-2.1 R (Livet et al., ) was purchased from Addgene (Cambridge, MA; plasmid 18723) and contains four cassettes of fluorescent protein (hrGFPII-NLS, eYFP, tdimer2, and M-mCerulean), each containing a loxp site linked to a fluorescent protein-coding region and a terminator. .. The Article Title: Development of an in vivo model to study clonal lineage relationships in hematopoietic cells using Brainbow2.1/Confetti mice Article Snippet: .. The four Brainbow2.1 XFPs were cloned from Expressing:Article Title: Development of an in vivo model to study clonal lineage relationships in hematopoietic cells using Brainbow2.1/Confetti mice Article Snippet: .. The four Brainbow2.1 XFPs were cloned from |